ModQuant workflow
Sample to signal in one working day.
A six-part workflow built around familiar molecular biology tools, clear checkpoints, and standard qPCR.
08:30
Prepare & normalize
09:15
Incubate
11:00
Capture & wash
12:30
qPCR setup
13:30
Readout
Step by step
A workflow your team can see at a glance.
Exact incubation conditions and reagent volumes belong in the approved user guide. This page helps teams understand the overall journey.
01
Bench setup
Prepare detector
Prepare the detector solution and required controls according to the user guide.
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02
Sample prep
Normalize input
Bring each endogenous mRNA sample into the 1–10 ng working range.
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03
Thermal cycler
Incubate
Combine sample and detector chemistry through a guided thermal step.
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04
Magnetic tool
Capture
Use magnetic beads to capture the detector-associated signal.
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05
Bench step
Wash & elute
Remove background and recover enriched material for amplification.
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06
qPCR
Quantify
Run real-time PCR and calculate the relative m⁶A signal across samples.
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Magnetic capture
Choose the tool that matches your throughput.
Use the tube magnet for focused, low-throughput work or the 96-well plate magnet to support broader sample sets.
Before you begin
Plan the input.
- Prepare endogenous mRNA
- Confirm 1–10 ng working range
- Select 24 or 48 reactions
- Choose tube or plate magnet
Equipment
Use familiar tools.
- Standard thermal cycler
- Real-time PCR instrument
- Magnetic separation tool
- General molecular biology equipment
Storage
Protect the chemistry.
- −20°C components
- 4°C components
- Refer to approved product labels
- Research Use Only
Ready to plan a run?